Publication: Synthesis and characterization of a new fluorescent probe for reactive oxygen species

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Title	Synthesis and characterization of a new fluorescent probe for reactive oxygen species
Authors/Editors*	Belinda Heyne, Chad Beddie and J. C. Scaiano
Where published*	Organic and Biomolecular Chemistry
How published*	Journal
Year*	2007
Volume	5
Number
Pages	1454 - 1458
Publisher	RSC Publishings
Keywords	fluorescence, reactive oxygen species, radical chemistry
Link	http://www.rsc.org/publishing/journals/OB/article.asp?doi=b702618h
Abstract	We report the development of a new fluorescence sensor for reactive oxygen species (ROS) based on a benzofurazan structure. The sensor, NBFhd, is initially non-fluorescent and reacts with peroxyl radicals by hydrogen transfer in an aqueous medium under neutral conditions to release the fluorescent N-methyl-4-amino-7-nitrobenzofurazan (NBF) and 1,4-benzoquinone. NBFhd shows excellent contrast and no interference in the region of cell autofluorescence and is a new tool to detect ROS in homogeneous and biological systems.